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flag control (Addgene inc)

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Structured Review

Addgene inc flag control
Flag Control, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+flag+ha+plasmid/FLAG-HA-pcDNA3%2E1-+(Plasmid+%2352535)/pm41838122-98-5-7
Average 95 stars, based on 115 article reviews

flag control - by Bioz Stars, 2026-10

95/100 stars

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Transfection:

Article Title: The ABL2 kinase regulates an HSF1-dependent transcriptional program required for lung adenocarcinoma brain metastasis
Article Snippet: .. Transient transfections and site-directed mutagenesis The pcDNA-hHSF1 WT plasmid was a gift from Lea Sistonen (Addgene plasmid # 71724; http://n2t.net/addgene:71724; RRID:Addgene_71724) (12) and the empty vector pcDNA3 Flag HA plasmid was a gift from William Sellers (Addgene plasmid # 10792; http://n2t.net/addgene:10792; RRID:Addgene_10792). ..

Mutagenesis:

Plasmid Preparation:

Article Title: AKT1 mediates multiple phosphorylation events that functionally promote HSF1 activation
Article Snippet: The FLAG-HSF-1 plasmid was purchased from Addgene (ID 32537, RRID:Addgene_32537), which was originally established by Dr Stuart Calderwood [ ]. .. The pcDNA3 Flag HA plasmid was purchased from Addgene (ID 10792, RRID:Addgene_10792), which was originally established by Dr William Sellers. .. The pcDNA3 Myr HA AKT1 plasmid was purchased from Addgene (ID 9008, RRID:Addgene_9008), which was originally established by Dr William Sellers [ ].

Article Title: Prolactin receptor localization and dynamics: Insights from quantitative imaging and mathematical modeling
Article Snippet: A plasmid containing human prolactin receptor (hPRLR), fused to the rat PRLR signal peptide, was obtained as a kind gift from Vincent Goffin (INSERM) ( ). .. The pcDNA3 FLAG HA plasmid was obtained as a gift from William Sellers (Addgene plasmid #10792; http://n2t.net/addgene:10792 ; RRID:Addgene_10792). .. A signal peptide (rat PRLR, human PRLR, or human CD86 signal peptide), hPRLR, and a yellow-green fluorescent protein (mNeonGreen, and, separately mEGFP) are cloned into the pcDNA3 plasmid using Takara In-Fusion Snap Assembly (#638947) according to the manufacturer’s protocol.

Article Title: AKT1 mediates multiple phosphorylation events that functionally promote HSF1 activation
Article Snippet: The FLAG-HSF-1 plasmid was purchased from Addgene (ID 32537, RRID:Addgene_32537), which was originally established by Dr Stuart Calderwood ( ). .. The pcDNA3 Flag HA plasmid was purchased from Addgene (ID 10792, RRID:Addgene_10792), which was originally established by Dr William Sellers. .. The pcDNA3 Myr HA AKT1 plasmid was purchased from Addgene (ID 9008, RRID:Addgene_9008), which was originally established by Dr William Sellers ( ).